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E101 · Colours

Riboflavin

No known risk colournatural origin

Vitamin B2 used as color.

Category
Colours
Vegan
maybe
Vegetarian
Yes

What it is

Riboflavin (E101) is Vitamin B2. Aside from being an essential human nutrient, it naturally possesses a bright, fluorescent yellow-orange color, making it an excellent natural food dye.

How it is used in food

Acts as both a colorant and a vitamin (Vitamin B2).

Commonly found in
  • Breakfast cereals (as both color and fortification)
  • Energy drinks and vitamin waters
  • Sauces and processed cheeses

Health and safety

None known.

Widely recognized as safe and essential for human health.

Regulatory status

RegionStatusNotes
Australia & New Zealand
FSANZ
Permitted Permitted.
European Union
EFSA / European Commission
Permitted Authorised for food use under EU regulations (Annex II).
United States
FDA (Food and Drug Administration)
Permitted GRAS.

Identification on labels

E-number
E101

Also printed asLactoflavin · Vitamin B2

Alternatives to this additive

  • E100: Curcumin — A plant-based natural yellow, though it lacks the nutritional benefit of Vitamin B2.

How it is made

While it can be synthesized chemically, almost all commercial E101 is produced naturally via fermentation by bacteria or fungi (like Bacillus subtilis).

  1. Fermentation — Produced via fermentation by specialized fungi or bacteria.
  2. Purification — The riboflavin is crystallized and separated from the fermentation broth.

Worth knowing

It is the same riboflavin your body uses to convert food into energy.

Conclusion

Seeing E101 on an ingredient label is actually a positive sign. It is a completely harmless, beneficial nutrient being used creatively to avoid synthetic chemical dyes.

References

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Entry last reviewed 2026-07-28.

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